nebuilder hifi dna assembly cloning kit (New England Biolabs)
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New England Biolabs
nebuilder hifi dna assembly cloning kit
Nebuilder Hifi Dna Assembly Cloning Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3084 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e5520s/NEBuilder+HiFi+DNA+Assembly+Cloning+Kit/pmc12811641-347-15-21
Average 99 stars, based on 3084 article reviews
Nebuilder Hifi Dna Assembly Cloning Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3084 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e5520s/NEBuilder+HiFi+DNA+Assembly+Cloning+Kit/pmc12811641-347-15-21
Average 99 stars, based on 3084 article reviews
nebuilder hifi dna assembly cloning kit - by Bioz Stars,
2026-09
99/100 stars
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Polymerase Chain Reaction:Article Title: Calponin isoforms define the cell-type-specific organization and dynamics of actomyosin bundles. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Calponin-1 Abcam Cat# ab46794 (EP798Y) Rabbit anti-Calponin-2 Proteintech Cat# 21073-1AP Mouse anti-Calponin-3 Santacruz Cat# SC271188 Mouse anti-Vinculin Sigma-Aldrich Cat# V9131 Rabbit anti-NM-IIA BioLegend Cat# 909801 Mouse anti-α-actinin-1 Sigma-Aldrich Cat# A5044 Rabbit anti-GAPDH Sigma-Aldrich Cat# G9545 Bacterial and virus strains E. coli XL10 gold ultracompetent cells This study N/A Chemicals, peptides, and recombinant proteins Alexa Fluor 488, 568 and 647 conjugated to phalloidin Thermo Fisher Scientific Cat# A12379, Cat# A1238, and Cat# A22287 Secondary antibodies conjugated to Alexa Fluor 488, 568 and 647 Thermo Fisher Scientific Cat# A11001, Cat# A11004, Cat# A31571, Cat# A11034, and Cat# A21245 4’,6-diamidino-2-phenylindole, dihydrochloride (DAPI) Thermo Fisher Scientific Cat# D1306 HRP-conjugated goat anti–rabbit Thermo Fisher Scientific Cat# G21234 HRP-conjugated goat anti–mouse Thermo Fisher Scientific Cat# 31430 Cell Mask Deep red Thermo Fisher Scientific Cat # H32717 Epidermal growth factor Peprotech Cat# AF-100-15 Fibronectin Merck Cat# 1080938001 ProLong Glass Antifade mountant Invitrogen Cat# P36980 Vectashield Vibrance antifade mounting medium Vector Laboratories Cat# H-1700-10 Dimethyl sulfoxide (DMSO) Sigma Cat# D2650-5X10ML Dulbecco’s modified Eagle’s medium (DMEM) Euro Clone Cat# ECB7501L Fetal Bovine Serum (FBS) GIBCO Cat# 10500-064 10 U/ml penicillin, 10 mg/ml streptomycin, and 20 mM L-glutamine GIBCO Cat# 10378-016 FluoroBrite DMEM GIBCO Cat# A1896701 PLL-g-PEG Surface technology N/A Silane Sigma Cat# GE17-1330-01 200 nm Yellow-Green fluorescent beads Thermo Fisher Scientific Cat# F8811 Acrylamide Sigma Cat# A4058 N,N’-methylenebisacrylamide Sigma Cat# M1533 Ammonium persulfate Bio-Rad Cat# 1610700 N,N,N’N’-tetramethylethylenediamine Sigma Cat# T9281 Sulfo-SANPAH Sigma Cat# 803332 N-(3-Dimethylaminopropyl)-N ′ - ethylcarbodiimide hydrochloride Sigma Cat# 03450 Critical commercial assays Mycoalert Mycoplasma Detection Kit LONZA Cat# LT07-418 FuGENE HD Promega Cat# E2312 jetPRIME (Polyplus transfection) Biotop Cat# 409-10 PureLink Genomic DNA Mini Kit Thermo Fisher Scientific Cat# K182001 (Continued on next page) Current Biology 35, 1–14.e1–e8, December 15, 2025 e1 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER NucleoSpin Gel and PCR Clean-up Macherey-Nagel, Vingmed Cat# 740609.250 NucleoSpin Plasmid EasyPure Macherey-Nagel, Vingmed Cat# 740727.250 NEBuilder kit New England BioLabs Cat# Plasmid Preparation:Article Title: Calponin isoforms define the cell-type-specific organization and dynamics of actomyosin bundles. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Calponin-1 Abcam Cat# ab46794 (EP798Y) Rabbit anti-Calponin-2 Proteintech Cat# 21073-1AP Mouse anti-Calponin-3 Santacruz Cat# SC271188 Mouse anti-Vinculin Sigma-Aldrich Cat# V9131 Rabbit anti-NM-IIA BioLegend Cat# 909801 Mouse anti-α-actinin-1 Sigma-Aldrich Cat# A5044 Rabbit anti-GAPDH Sigma-Aldrich Cat# G9545 Bacterial and virus strains E. coli XL10 gold ultracompetent cells This study N/A Chemicals, peptides, and recombinant proteins Alexa Fluor 488, 568 and 647 conjugated to phalloidin Thermo Fisher Scientific Cat# A12379, Cat# A1238, and Cat# A22287 Secondary antibodies conjugated to Alexa Fluor 488, 568 and 647 Thermo Fisher Scientific Cat# A11001, Cat# A11004, Cat# A31571, Cat# A11034, and Cat# A21245 4’,6-diamidino-2-phenylindole, dihydrochloride (DAPI) Thermo Fisher Scientific Cat# D1306 HRP-conjugated goat anti–rabbit Thermo Fisher Scientific Cat# G21234 HRP-conjugated goat anti–mouse Thermo Fisher Scientific Cat# 31430 Cell Mask Deep red Thermo Fisher Scientific Cat # H32717 Epidermal growth factor Peprotech Cat# AF-100-15 Fibronectin Merck Cat# 1080938001 ProLong Glass Antifade mountant Invitrogen Cat# P36980 Vectashield Vibrance antifade mounting medium Vector Laboratories Cat# H-1700-10 Dimethyl sulfoxide (DMSO) Sigma Cat# D2650-5X10ML Dulbecco’s modified Eagle’s medium (DMEM) Euro Clone Cat# ECB7501L Fetal Bovine Serum (FBS) GIBCO Cat# 10500-064 10 U/ml penicillin, 10 mg/ml streptomycin, and 20 mM L-glutamine GIBCO Cat# 10378-016 FluoroBrite DMEM GIBCO Cat# A1896701 PLL-g-PEG Surface technology N/A Silane Sigma Cat# GE17-1330-01 200 nm Yellow-Green fluorescent beads Thermo Fisher Scientific Cat# F8811 Acrylamide Sigma Cat# A4058 N,N’-methylenebisacrylamide Sigma Cat# M1533 Ammonium persulfate Bio-Rad Cat# 1610700 N,N,N’N’-tetramethylethylenediamine Sigma Cat# T9281 Sulfo-SANPAH Sigma Cat# 803332 N-(3-Dimethylaminopropyl)-N ′ - ethylcarbodiimide hydrochloride Sigma Cat# 03450 Critical commercial assays Mycoalert Mycoplasma Detection Kit LONZA Cat# LT07-418 FuGENE HD Promega Cat# E2312 jetPRIME (Polyplus transfection) Biotop Cat# 409-10 PureLink Genomic DNA Mini Kit Thermo Fisher Scientific Cat# K182001 (Continued on next page) Current Biology 35, 1–14.e1–e8, December 15, 2025 e1 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER NucleoSpin Gel and PCR Clean-up Macherey-Nagel, Vingmed Cat# 740609.250 NucleoSpin Plasmid EasyPure Macherey-Nagel, Vingmed Cat# 740727.250 NEBuilder kit New England BioLabs Cat# SDS Page:Article Title: Calponin isoforms define the cell-type-specific organization and dynamics of actomyosin bundles. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Calponin-1 Abcam Cat# ab46794 (EP798Y) Rabbit anti-Calponin-2 Proteintech Cat# 21073-1AP Mouse anti-Calponin-3 Santacruz Cat# SC271188 Mouse anti-Vinculin Sigma-Aldrich Cat# V9131 Rabbit anti-NM-IIA BioLegend Cat# 909801 Mouse anti-α-actinin-1 Sigma-Aldrich Cat# A5044 Rabbit anti-GAPDH Sigma-Aldrich Cat# G9545 Bacterial and virus strains E. coli XL10 gold ultracompetent cells This study N/A Chemicals, peptides, and recombinant proteins Alexa Fluor 488, 568 and 647 conjugated to phalloidin Thermo Fisher Scientific Cat# A12379, Cat# A1238, and Cat# A22287 Secondary antibodies conjugated to Alexa Fluor 488, 568 and 647 Thermo Fisher Scientific Cat# A11001, Cat# A11004, Cat# A31571, Cat# A11034, and Cat# A21245 4’,6-diamidino-2-phenylindole, dihydrochloride (DAPI) Thermo Fisher Scientific Cat# D1306 HRP-conjugated goat anti–rabbit Thermo Fisher Scientific Cat# G21234 HRP-conjugated goat anti–mouse Thermo Fisher Scientific Cat# 31430 Cell Mask Deep red Thermo Fisher Scientific Cat # H32717 Epidermal growth factor Peprotech Cat# AF-100-15 Fibronectin Merck Cat# 1080938001 ProLong Glass Antifade mountant Invitrogen Cat# P36980 Vectashield Vibrance antifade mounting medium Vector Laboratories Cat# H-1700-10 Dimethyl sulfoxide (DMSO) Sigma Cat# D2650-5X10ML Dulbecco’s modified Eagle’s medium (DMEM) Euro Clone Cat# ECB7501L Fetal Bovine Serum (FBS) GIBCO Cat# 10500-064 10 U/ml penicillin, 10 mg/ml streptomycin, and 20 mM L-glutamine GIBCO Cat# 10378-016 FluoroBrite DMEM GIBCO Cat# A1896701 PLL-g-PEG Surface technology N/A Silane Sigma Cat# GE17-1330-01 200 nm Yellow-Green fluorescent beads Thermo Fisher Scientific Cat# F8811 Acrylamide Sigma Cat# A4058 N,N’-methylenebisacrylamide Sigma Cat# M1533 Ammonium persulfate Bio-Rad Cat# 1610700 N,N,N’N’-tetramethylethylenediamine Sigma Cat# T9281 Sulfo-SANPAH Sigma Cat# 803332 N-(3-Dimethylaminopropyl)-N ′ - ethylcarbodiimide hydrochloride Sigma Cat# 03450 Critical commercial assays Mycoalert Mycoplasma Detection Kit LONZA Cat# LT07-418 FuGENE HD Promega Cat# E2312 jetPRIME (Polyplus transfection) Biotop Cat# 409-10 PureLink Genomic DNA Mini Kit Thermo Fisher Scientific Cat# K182001 (Continued on next page) Current Biology 35, 1–14.e1–e8, December 15, 2025 e1 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER NucleoSpin Gel and PCR Clean-up Macherey-Nagel, Vingmed Cat# 740609.250 NucleoSpin Plasmid EasyPure Macherey-Nagel, Vingmed Cat# 740727.250 NEBuilder kit New England BioLabs Cat# Recombinant:Article Title: Calponin isoforms define the cell-type-specific organization and dynamics of actomyosin bundles. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Calponin-1 Abcam Cat# ab46794 (EP798Y) Rabbit anti-Calponin-2 Proteintech Cat# 21073-1AP Mouse anti-Calponin-3 Santacruz Cat# SC271188 Mouse anti-Vinculin Sigma-Aldrich Cat# V9131 Rabbit anti-NM-IIA BioLegend Cat# 909801 Mouse anti-α-actinin-1 Sigma-Aldrich Cat# A5044 Rabbit anti-GAPDH Sigma-Aldrich Cat# G9545 Bacterial and virus strains E. coli XL10 gold ultracompetent cells This study N/A Chemicals, peptides, and recombinant proteins Alexa Fluor 488, 568 and 647 conjugated to phalloidin Thermo Fisher Scientific Cat# A12379, Cat# A1238, and Cat# A22287 Secondary antibodies conjugated to Alexa Fluor 488, 568 and 647 Thermo Fisher Scientific Cat# A11001, Cat# A11004, Cat# A31571, Cat# A11034, and Cat# A21245 4’,6-diamidino-2-phenylindole, dihydrochloride (DAPI) Thermo Fisher Scientific Cat# D1306 HRP-conjugated goat anti–rabbit Thermo Fisher Scientific Cat# G21234 HRP-conjugated goat anti–mouse Thermo Fisher Scientific Cat# 31430 Cell Mask Deep red Thermo Fisher Scientific Cat # H32717 Epidermal growth factor Peprotech Cat# AF-100-15 Fibronectin Merck Cat# 1080938001 ProLong Glass Antifade mountant Invitrogen Cat# P36980 Vectashield Vibrance antifade mounting medium Vector Laboratories Cat# H-1700-10 Dimethyl sulfoxide (DMSO) Sigma Cat# D2650-5X10ML Dulbecco’s modified Eagle’s medium (DMEM) Euro Clone Cat# ECB7501L Fetal Bovine Serum (FBS) GIBCO Cat# 10500-064 10 U/ml penicillin, 10 mg/ml streptomycin, and 20 mM L-glutamine GIBCO Cat# 10378-016 FluoroBrite DMEM GIBCO Cat# A1896701 PLL-g-PEG Surface technology N/A Silane Sigma Cat# GE17-1330-01 200 nm Yellow-Green fluorescent beads Thermo Fisher Scientific Cat# F8811 Acrylamide Sigma Cat# A4058 N,N’-methylenebisacrylamide Sigma Cat# M1533 Ammonium persulfate Bio-Rad Cat# 1610700 N,N,N’N’-tetramethylethylenediamine Sigma Cat# T9281 Sulfo-SANPAH Sigma Cat# 803332 N-(3-Dimethylaminopropyl)-N ′ - ethylcarbodiimide hydrochloride Sigma Cat# 03450 Critical commercial assays Mycoalert Mycoplasma Detection Kit LONZA Cat# LT07-418 FuGENE HD Promega Cat# E2312 jetPRIME (Polyplus transfection) Biotop Cat# 409-10 PureLink Genomic DNA Mini Kit Thermo Fisher Scientific Cat# K182001 (Continued on next page) Current Biology 35, 1–14.e1–e8, December 15, 2025 e1 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER NucleoSpin Gel and PCR Clean-up Macherey-Nagel, Vingmed Cat# 740609.250 NucleoSpin Plasmid EasyPure Macherey-Nagel, Vingmed Cat# 740727.250 NEBuilder kit New England BioLabs Cat# Cloning:Article Title: Mycobacterium tuberculosis triggers reduced inflammatory cytokine responses and virulence in mice lacking Tax1bp1 Article Snippet: Commercial kit , Q5 Site Directed Mutagenesis kit , New England Biolabs , E0554S , . .. Commercial kit , NEBuilder HiFi DNA Assembly Cloning Kit , New England Biolabs , Article Title: Integrins mediate symbiont-specific uptake in cnidarian larvae Article Snippet: TOP10 chemically competent E. coli , ThermoFisher Scientific , C404010. .. 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Megawhop NE builder Hifi DNA assembly cloning kit , New England Biolabs , other:Article Title: Antibiotic potentiation and inhibition of cross-resistance in pathogens associated with cystic fibrosis Article Snippet: Commercial |